Melike Caglayan
Address:
University of Florida, Department of Biochemistry and Molecular Biology.
Gainesville,FL 32610.
Research Interests:
- Oxidative Stress
- Genome Maintenance
- DNA Repair
- Nucleic Acids Enzymology
Biography:
Dr. Caglayan's Ph.D. work focused on investigating the temperature effect on the fidelity of bacterial DNA replication and DNA polymerase transient state kinetics. In 2013, she joined the laboratory of Dr. Samuel H. Wilson at National Institutes of Health (NIH), National Institutes of Environmental Health (NIEHS), to study the base excision DNA repair by characterizing nucleic acid-protein and protein-protein interactions and their role in coordinating the sequential enzymatic steps of the DNA repair pathway. Dr. Caglayan joined the faculty at the University of Florida in June 2018.
Publications:
1) Caglayan M. and Wilson S.H. Pol μ dGTP mismatch insertion opposite T coupled with ligation reveals a
promutagenic DNA intermediate during double strand break repair. Nature Communications (2018) 9:4213.
2) Horton J.K., Stefanick D.F., Caglayan M., Zhao M.L., Gassman N.R., Wilson S.H. XRCC1 phosphorylation
affects aprataxin recruitment and DNA deadenylation activity. DNA Repair (2018)– 64:26– 33.
3) Prasad R., Caglayan M., Da-Peng D.,Nadalutti C.A., Gassman N.R., Zhao M., Stefanick D.F.,Horton J.K.,
Krasich R., Longley M.J., Copeland W.C.,Griffith J.D., WilsonS.H. DNA polymerase β: The missing link of the
base excision repair machinery in mammalian mitochondria. DNA Repair (2017) 60: 77 – 88.
4) Caglayan M., Prasad R., Krasich R., Longley M.J., Kadoda K.,Tsuda M.,Sasanuma H.,Takeda S.,Tano K.,
Copeland W.C., Wilson S.H.Complementation of aprataxin deficiency by base excision repair enzymes in
mitochondrial extracts.Nucleic Acids Research (2017) 17:10079– 10088.
5) Caglayan M., Wilson S.H.Role of DNA polymerase βoxidized nucleotide insertion in DNA ligation failure.
Journal of Radiation Research - Review article (2017)1093: 1 – 5.
6) Caglayan M., Horton J.K., Da-Peng D., Stefanick D.F., Wilson S.H. Oxidized nucleotide insertion by pol β
confounds ligation during base excision repair. Nature Communications (2017) 8: 14045.
7) Sassa A., Caglayan M.,Rodriguez Y.,Beard W.A., Wilson S.H.,Nohmi T.,Honma M.,Yasui M.Impact of
ribonucleotide backbone on translesion synthesis andrepair of 7,8-Dihydro-8-oxoguanine. Journal of
Biological Chemistry (2016) 291: 24314 – 24323.
8) Caglayan M., Wilson S.H.Oxidant and environmental toxicant-induced effects compromise DNA ligation
during base excision DNA repair. DNA Repair - Review article (2015) 35:85– 89.
9) Caglayan M., Horton J.K.,Prasad R.,Wilson S.H.Complementation of aprataxin deficiency by base excision repair enzymes. Nucleic Acids Research (2015) 43:2271– 2281.
10) Caglayan M.,Batra V.K.,Sassa A.,Prasad R.,WilsonS.H. Role of polymerase β in complementing aprataxin
deficiency during abasic-site base excision repair. Nature Structural and Molecular Biology (2014) 21:497–499.
